Journal: Nature Communications

Article Title: Adipocyte OGT governs diet-induced hyperphagia and obesity

doi: 10.1038/s41467-018-07461-x

Figure Lengend Snippet: Adipocyte OGT drives diet-induced hyperphagia and obesity. a Genetic cross scheme. b Growth curves and c body composition of adipocyte-specific OGT knockout mice (FKO) and littermate control mice (Con) on chow ( n = 8 (Con, dashed line with empty square/stripped bar) or 11 (FKO, blue line/bar)) or HFD ( n = 11 (Con, dashed line with empty circle/empty bar) or 13 (FKO, red line/bar)). d Circulating levels of leptin and e wet tissue weights in male Con ( n = 6) and FKO ( n = 6) mice fed HFD for 9 weeks, or age-matched Con ( n = 8) and FKO ( n = 8) mice fed chow. BAT, interscapular brown adipose tissue. White adipose tissues include depots from perigonadal (pg), subcutaneous (sc), and retroperitoneal (rp) regions. f Energy intake of male mice on HFD ( n = 7 per genotype) or Chow ( n = 5 (Con) or n = 6 (FKO)) for 13 weeks. g Fecal energy content. h Daily energy intake. Male Con ( n = 10) and FKO ( n = 10) mice fed HFD for 6 weeks. i Energy expenditure of 5-week HFD-fed male Con ( n = 7) and FKO ( n = 9) mice. j Energy intake in a two-choice diet preference test. Male Con ( n = 9) and FKO ( n = 10) mice have been fed HFD for 7 or 16 weeks. k Growth curves of HFD-fed mice. Con (AL, ad libitum, n = 11), Con (PF, pair-fed to littermate FKO, n = 7), FKO (AL, n = 7). Data were presented as mean ± s.e.m. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, post hoc Sidak’s tests ( b (HFD Con vs. HFD FKO), i , k (Con PF vs. FKO AL)), Tukey’s tests ( c , d , f ), or two-tailed Student’s t-test ( e , g , h , j )

Article Snippet: Recombinant mouse leptin protein (5 mg/kg, R&D Systems cat# 498-OB) was reconstituted at 1 mg/mL in sterile 20 mM Tris-HCl, pH 8.0.

Techniques: Knock-Out, Control, Two Tailed Test

Journal: Nature Communications

Article Title: Adipocyte OGT governs diet-induced hyperphagia and obesity

doi: 10.1038/s41467-018-07461-x

Figure Lengend Snippet: Adipocyte OGT drives diet-induced hyperphagia through transactivation of lipid desaturation in adipose tissue. a Gene expression analysis of de novo lipid desaturation and synthesis in adipose tissue (Chow for 15 weeks, Con n = 8, FKO n = 8; age-matched HFD for 9 weeks, Con n = 5, FKO n = 6). b Protein levels of SCD and OGT in adipose tissue. c Western blotting analysis in adipose tissue from 9-week-HFD-fed FKO and Con mice (Con n = 5, FKO n = 6, two representative biological replicates were shown). d Growth curves and e energy intake of (m)HFD-fed Con and FKO mice ( n = 8–10 per group). mHFD denotes a mono-unsaturated fat-fortified high-fat diet that changes the saturated fat-rich soybean oil fraction into the mono-unsaturated fat-rich canola oil as illustrated in the diagram ( d ). f Contents of palmitate (C16:0) and palmitoleate (C16:1) DAG in adipose lipidome, and circulating levels of leptin ( g ), or of FGF21 ( h ) in mice fed HFD or mHFD for 15 weeks. Data were presented as mean ± s.e.m. n.s. not significant, * P < 0.05, ** P < 0.01, **** P < 0.0001, post hoc Sidak’s tests ( a , e – h ), or # P < 0.01 HFD Con vs. HFD FKO, two-tailed Student’s t-test ( a ). A dashed line in ( e – h ) indicates that data from HFD study and mHFD study are presented in a side-by-side manner, and inference may not be made between different diets

Article Snippet: Recombinant mouse leptin protein (5 mg/kg, R&D Systems cat# 498-OB) was reconstituted at 1 mg/mL in sterile 20 mM Tris-HCl, pH 8.0.

Techniques: Gene Expression, Western Blot, Two Tailed Test

Journal: The Journal of veterinary medical science

Article Title: Sandwich enzyme-linked immunosorbent assay of canine leptin.

doi: 10.1292/jvms.62.207

Figure Lengend Snippet: Fig. 1. SDS-PAGE and Western blot analyses of canine leptin. Recombinant canine (D), rat (R), mouse (M) and human (H) leptins (200 ng/lane) were electrophoresed through SDS-polyacrylamide gel (5–20%), and detected by either Coomassie brilliant blue R250 (top panel), anti- canine leptin antibody (second panel), anti-human leptin antibody (third panel), or anti-mouse leptin antibody (bottom panel).

Article Snippet: SDS-polyacrylamide gel (5–20%) electrophoresis (SDSPAGE) analysis revealed that the recombinant canine leptin migrated similarly to recombinant leptins of rat (R&D Systems, Minneapolis, MN, U.S.A.), mouse (PeproTechEC Ltd., London, England) and human (PeproTechEC), at the position of about 16 kDa (Fig. 1, top panel).

Techniques: SDS Page, Western Blot, Recombinant

Journal: The Journal of veterinary medical science

Article Title: Sandwich enzyme-linked immunosorbent assay of canine leptin.

doi: 10.1292/jvms.62.207

Figure Lengend Snippet: Fig. 2. Standard curve of sandwich ELISA using recombinant canine leptin. Recombinant canine leptin (0.5–32 ng/ml) was measured by a sandwich ELISA using purified anti-canine leptin antibody.

Article Snippet: SDS-polyacrylamide gel (5–20%) electrophoresis (SDSPAGE) analysis revealed that the recombinant canine leptin migrated similarly to recombinant leptins of rat (R&D Systems, Minneapolis, MN, U.S.A.), mouse (PeproTechEC Ltd., London, England) and human (PeproTechEC), at the position of about 16 kDa (Fig. 1, top panel).

Techniques: Sandwich ELISA, Recombinant, Purification

Journal: Heliyon

Article Title: Hyperleptinemia results in systemic inflammation and the exacerbation of ischemia-reperfusion myocardial injury.

doi: 10.1016/j.heliyon.2021.e08491

Figure Lengend Snippet: Figure 4. Myocardium staining with haematoxylin-eosin. Scale bar, 50 μm (400). (A) Control group; (B) group with chronic leptin administration; (C) group with chronic administration of JAK/STAT inhibitor – JSI-124 (Cucurbitacin-I); (D) group with chronic administration of leptin and JSI-124.

Article Snippet: Recombinant rat leptin (endotoxin level <0.10 EU per 1 μg of protein by the Limulus amoebocyte lysate method) (R&D Systems, Minneapolis, MN, USA) was reconstituted at 1 mg/mL in a sterile vehicle: 20 mM TrisHCI, pH 8.0.

Techniques: Staining, Control

Journal: American journal of physiology. Gastrointestinal and liver physiology

Article Title: Leptin regulates gallbladder genes related to absorption and secretion.

doi: 10.1152/ajpgi.00389.2006

Figure Lengend Snippet: Fig. 1. Leptin administration decreased gallbladder volume, bile sodium concentration, and pH.

Article Snippet: For leptin replacement, mice received daily intraperitoneal injections of either recombinant murine leptin (R&D Systems, Minneapolis, MN) at a dose of 5 g/g body wt or saline as a control for 4 wk.

Techniques: Concentration Assay

Journal: Biomaterials

Article Title: Amino acid-based compound activates atypical PKC and leptin receptor pathways to improve glycemia and anxiety like behavior in diabetic mice.

doi: 10.1016/j.biomaterials.2020.119839

Figure Lengend Snippet: Fig. 4. AAC2 induced glucose uptake by LepR-dependent mechanism in vivo. (a) GTT in Leprdb mice treated without (Veh, open circles, n = 6) or with AAC2 (black circles, n = 6). Student's independent t-test for each time point. P = 0.006 at 30 min post glucose injection. (b,c) Food intake, P = 0.817, ns. (b) and weight gain P = 0.576, ns. (c) in Leprdb mice treated without (Veh, open bars, n = 6) or with AAC2 (black bar, n = 6). (d) Insulin levels in plasma of same Leprdb mice were measured by ELISA. P = 0.416, ns. (e) GTT in Lepob treated without (Veh, open circles, n = 5) or with AAC2 (black circles, n = 5). Student's independent t-test for each time point. P = 0.001 and P = 0.045 at 5 min and 30 min post glucose injection, respectively. (f–h) Food intake, P = 0.133, ns. (f), weight gain (g), and insulin levels (h) in Lepob mice treated without (Veh, open bar, n = 5) or with AAC2 (black bar, n = 5) P = 0.17, ns. Student's independent t-test.

Article Snippet: Interaction of either AAC2 (0.1 μM in PBS) or mouse recombinant leptin protein (mLep; 1.6 fM in PBS; Crystal Chem, Elk Grove Village, IL, 90030-B) and with mouse recombinant leptin receptor (LepR) protein (1.6 pM in PBS; R&D systems, 497-LR/CF) were investigated by applying an alternating current on quartz via the piezoelectric effect using QCM-D [52,53].

Techniques: In Vivo, Injection, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

Journal: Endocrinology

Article Title: Leptin enhances human beta-defensin-2 production in human keratinocytes.

doi: 10.1210/en.2008-0343

Figure Lengend Snippet: FIG. 1. mRNA expression of the leptin receptor Ob-Rb in keratino- cytes (KC) incubated for 12 h with or without IL-1 (1 ng/ml). The cDNA of human peripheral blood mononuclear cells (PBMC) was used as the positive control for Ob-Rb. The results shown are representa- tive of four separate experiments.

Article Snippet: Recombinant human leptin, human leptin receptor/Fc chimera, which is composed of the leptin receptor extracellular domain fused to human IgG1 Fc, and control human IgG1 Fc were purchased from R&D Systems (Minneapolis, MN). c-Jun N-terminal kinase (JNK) inhibitor SP600125 was purchased from Biomol (Plymouth Meeting, MA). p38 MAPK inhibitor SB202190, MAPK/ERK kinase (MEK) inhibitor PD98059, and Janus kinase (JAK) 2 inhibitor AG490 were from Calbiochem (La Jolla, CA).

Techniques: Expressing, Incubation, Positive Control

Journal: Endocrinology

Article Title: Leptin enhances human beta-defensin-2 production in human keratinocytes.

doi: 10.1210/en.2008-0343

Figure Lengend Snippet: FIG. 2. Stimulation of IL-1-induced hBD-2 secretion by leptin (A and B) and mRNA expression (C). A, Keratinocytes were incubated with either the medium alone or with IL-1 (1 ng/ml) in the presence of the indicated concentrations of leptin. At 48 h, the amount of hBD-2 secreted was analyzed. The data in A are presented as the mean SEM of triplicate cultures and represent the data from four experiments. *, P 0.05 vs. the controls; †, P 0.05 vs. IL-1 alone. B and C, Keratinocytes were preincubated with 1 g/ml of the leptin receptor/ IgG1 Fc chimera (LR) or control human IgG1 Fc (Fc) for 30 min and were then incubated with 1 ng/ml IL-1 in the presence or absence of 10 ng/ml leptin. At 48 h, hBD-2 secretion was analyzed (B), whereas at 12 h, the hBD-2 mRNA levels were analyzed by real-time RT-PCR (C). The data in B and C are presented as the mean SEM (n 4). *, P 0.05 vs. the controls; †, P 0.05 vs. IL-1 alone; ‡, P 0.05 vs. IL-1 plus leptin. In C, the hBD-2 mRNA levels were normalized to the mRNA levels of GAPDH; these are represented as fold induction relative to control keratinocytes treated with medium alone.

Article Snippet: Recombinant human leptin, human leptin receptor/Fc chimera, which is composed of the leptin receptor extracellular domain fused to human IgG1 Fc, and control human IgG1 Fc were purchased from R&D Systems (Minneapolis, MN). c-Jun N-terminal kinase (JNK) inhibitor SP600125 was purchased from Biomol (Plymouth Meeting, MA). p38 MAPK inhibitor SB202190, MAPK/ERK kinase (MEK) inhibitor PD98059, and Janus kinase (JAK) 2 inhibitor AG490 were from Calbiochem (La Jolla, CA).

Techniques: Expressing, Incubation, Control, Quantitative RT-PCR

Journal: Endocrinology

Article Title: Leptin enhances human beta-defensin-2 production in human keratinocytes.

doi: 10.1210/en.2008-0343

Figure Lengend Snippet: FIG. 3. The effects of antisense oligonucleotides on hBD-2 secretion (A) and mRNA expression (B). Keratinocytes were transfected with antisense oligonucleotides (ASO) against transcription fac- tors or control scrambled oligonucleotides (Con) (0.2 M each) and were treated with IL-1 (1 ng/ ml) in the presence or absence of leptin (10 ng/ml). At 48 h, the amount of hBD-2 secreted was ana- lyzed (A), whereas at 12 h, the mRNA levels were analyzed by real-time RT-PCR (B). *, P 0.05 vs. the controls; †, P 0.05 vs. IL-1; ‡, P 0.05 vs. IL-1 plus leptin. The data are presented as the mean SEM (n 4). In B, the hBD-2 mRNA levels were normalized to the mRNA levels of GAPDH; these are presented as fold induction.

Article Snippet: Recombinant human leptin, human leptin receptor/Fc chimera, which is composed of the leptin receptor extracellular domain fused to human IgG1 Fc, and control human IgG1 Fc were purchased from R&D Systems (Minneapolis, MN). c-Jun N-terminal kinase (JNK) inhibitor SP600125 was purchased from Biomol (Plymouth Meeting, MA). p38 MAPK inhibitor SB202190, MAPK/ERK kinase (MEK) inhibitor PD98059, and Janus kinase (JAK) 2 inhibitor AG490 were from Calbiochem (La Jolla, CA).

Techniques: Expressing, Transfection, Control, Quantitative RT-PCR

Journal: Endocrinology

Article Title: Leptin enhances human beta-defensin-2 production in human keratinocytes.

doi: 10.1210/en.2008-0343

Figure Lengend Snippet: FIG. 4. Stimulation of the basal and IL-1-induced transcriptional activities of NF-B (A), STAT1 (B), and STAT3 (C) by leptin. Kera- tinocytes transfected with pRL-tk and pNF-B-luc, pGAS-luc, or pSTAT3-luc were incubated with IL-1 (1 ng/ml) in the presence or absence of the indicated concentrations of leptin. At 18 h, the tran- scriptional activities were analyzed. *, P 0.05 vs. the controls with the medium alone. The data are represented as the mean SEM (n 4).

Article Snippet: Recombinant human leptin, human leptin receptor/Fc chimera, which is composed of the leptin receptor extracellular domain fused to human IgG1 Fc, and control human IgG1 Fc were purchased from R&D Systems (Minneapolis, MN). c-Jun N-terminal kinase (JNK) inhibitor SP600125 was purchased from Biomol (Plymouth Meeting, MA). p38 MAPK inhibitor SB202190, MAPK/ERK kinase (MEK) inhibitor PD98059, and Janus kinase (JAK) 2 inhibitor AG490 were from Calbiochem (La Jolla, CA).

Techniques: Transfection, Incubation

Journal: Endocrinology

Article Title: Leptin enhances human beta-defensin-2 production in human keratinocytes.

doi: 10.1210/en.2008-0343

Figure Lengend Snippet: FIG. 5. The effects of signal inhibitors on IL-1- and/or leptin-induced hBD-2 secretion (A) and mRNA expression (B). Keratinocytes were preincubated for 30 min with 25 M AG490 (AG), 10 M PD98059 (PD), 1 M SB202190 (SB), or 10 M SP600125 (SP), and were then incubated with 1 ng/ml IL-1 in the pres- ence or absence of 10 ng/ml leptin. At 48 h, hBD-2 secretion was analyzed (A), whereas at 12 h, hBD-2 mRNA levels were analyzed by real-time RT-PCR (B). The data represent the mean SEM (n 4). In B, hBD-2 mRNA levels were nor- malized to the mRNA levels of GAPDH and are shown as a fold induction. *, P 0.05 vs. the controls; †, P 0.05 vs. IL-1 alone; ‡, P 0.05 vs. IL-1 plus leptin.

Article Snippet: Recombinant human leptin, human leptin receptor/Fc chimera, which is composed of the leptin receptor extracellular domain fused to human IgG1 Fc, and control human IgG1 Fc were purchased from R&D Systems (Minneapolis, MN). c-Jun N-terminal kinase (JNK) inhibitor SP600125 was purchased from Biomol (Plymouth Meeting, MA). p38 MAPK inhibitor SB202190, MAPK/ERK kinase (MEK) inhibitor PD98059, and Janus kinase (JAK) 2 inhibitor AG490 were from Calbiochem (La Jolla, CA).

Techniques: Expressing, Incubation, Quantitative RT-PCR

Journal: Endocrinology

Article Title: Leptin enhances human beta-defensin-2 production in human keratinocytes.

doi: 10.1210/en.2008-0343

Figure Lengend Snippet: FIG. 7. IL-1 and/or leptin-induced phosphorylation of IB (A), ty- rosine- or serine-phosphorylation of STAT1 (B) and STAT3 (C). Ker- atinocytes were preincubated for 30 min with 25 M AG490 (AG) or 1 M SB202190 (SB), and were then treated with IL-1 (1 ng/ml) for 5 min in the presence or absence of leptin (10 ng/ml) to analyze the phosphorylation by Western blotting. The results represent the data from four separate experiments.

Article Snippet: Recombinant human leptin, human leptin receptor/Fc chimera, which is composed of the leptin receptor extracellular domain fused to human IgG1 Fc, and control human IgG1 Fc were purchased from R&D Systems (Minneapolis, MN). c-Jun N-terminal kinase (JNK) inhibitor SP600125 was purchased from Biomol (Plymouth Meeting, MA). p38 MAPK inhibitor SB202190, MAPK/ERK kinase (MEK) inhibitor PD98059, and Janus kinase (JAK) 2 inhibitor AG490 were from Calbiochem (La Jolla, CA).

Techniques: Phospho-proteomics, Western Blot

Journal: Endocrinology

Article Title: Leptin enhances human beta-defensin-2 production in human keratinocytes.

doi: 10.1210/en.2008-0343

Figure Lengend Snippet: FIG. 6. The effects of signal inhibitors on the activities of NF-B (A), STAT1 (B), and STAT3 (C). Keratinocytes transfected with pRL-tk and pNF-B-luc, pGAS-luc, or pSTAT3-luc were preincubated for 30 min with 25 M AG490 (AG) or 1 M SB202190 (SB), and were then incubated with 1 ng/ml IL-1 in the presence or absence of 10 ng/ml leptin. At 18 h, transcriptional activities were analyzed. *, P 0.05 vs. the controls; †, P 0.05 vs. IL-1 alone; ‡, P 0.05 vs. leptin alone; §, P 0.05 vs. IL-1 plus leptin. The data represent the mean SEM (n 4).

Article Snippet: Recombinant human leptin, human leptin receptor/Fc chimera, which is composed of the leptin receptor extracellular domain fused to human IgG1 Fc, and control human IgG1 Fc were purchased from R&D Systems (Minneapolis, MN). c-Jun N-terminal kinase (JNK) inhibitor SP600125 was purchased from Biomol (Plymouth Meeting, MA). p38 MAPK inhibitor SB202190, MAPK/ERK kinase (MEK) inhibitor PD98059, and Janus kinase (JAK) 2 inhibitor AG490 were from Calbiochem (La Jolla, CA).

Techniques: Transfection, Incubation

Journal: Endocrinology

Article Title: Leptin enhances human beta-defensin-2 production in human keratinocytes.

doi: 10.1210/en.2008-0343

Figure Lengend Snippet: FIG. 8. IL-1 and/or leptin-induced phosphorylation of JAK2 (A) and p38 MAPK (B). Keratinocytes were preincubated for 30 min with 25 M AG490 (AG) or 1 M SB202190 (SB), and were then treated for 5 min with IL-1 (1 ng/ml) in the presence or absence of leptin (10 ng/ml) to analyze the phosphorylation by Western blotting. The results rep- resent the data from four separate experiments.

Article Snippet: Recombinant human leptin, human leptin receptor/Fc chimera, which is composed of the leptin receptor extracellular domain fused to human IgG1 Fc, and control human IgG1 Fc were purchased from R&D Systems (Minneapolis, MN). c-Jun N-terminal kinase (JNK) inhibitor SP600125 was purchased from Biomol (Plymouth Meeting, MA). p38 MAPK inhibitor SB202190, MAPK/ERK kinase (MEK) inhibitor PD98059, and Janus kinase (JAK) 2 inhibitor AG490 were from Calbiochem (La Jolla, CA).

Techniques: Phospho-proteomics, Western Blot

Journal: Endocrinology

Article Title: Leptin enhances human beta-defensin-2 production in human keratinocytes.

doi: 10.1210/en.2008-0343

Figure Lengend Snippet: FIG. 9. A schematic model for the pathways in IL-1 plus leptin- induced hBD-2 expression. Black and white arrows indicate IL-1- induced and leptin-induced pathways, respectively. Ser-P, Serine phosphorylation; Tyr-P, tyrosine phosphorylation.

Article Snippet: Recombinant human leptin, human leptin receptor/Fc chimera, which is composed of the leptin receptor extracellular domain fused to human IgG1 Fc, and control human IgG1 Fc were purchased from R&D Systems (Minneapolis, MN). c-Jun N-terminal kinase (JNK) inhibitor SP600125 was purchased from Biomol (Plymouth Meeting, MA). p38 MAPK inhibitor SB202190, MAPK/ERK kinase (MEK) inhibitor PD98059, and Janus kinase (JAK) 2 inhibitor AG490 were from Calbiochem (La Jolla, CA).

Techniques: Expressing, Phospho-proteomics

Journal: Degenerative Neurological and Neuromuscular Disease

Article Title: Neuroprotective Effects of Leptin on the APP/PS1 Alzheimer’s Disease Mouse Model: Role of Microglial and Neuroinflammation

doi: 10.2147/dnnd.s427781

Figure Lengend Snippet: Figure 1 Influence of leptin on the levels of Aβ1-40 (Left) and Aβ1-42 (Right) in the adult + leptin group, adult + saline group, aged + leptin group and aged + saline group.

Article Snippet: Recombinant mouse leptin (Elabscience.

Techniques: Saline

Journal: Degenerative Neurological and Neuromuscular Disease

Article Title: Neuroprotective Effects of Leptin on the APP/PS1 Alzheimer’s Disease Mouse Model: Role of Microglial and Neuroinflammation

doi: 10.2147/dnnd.s427781

Figure Lengend Snippet: Figure 2 Western blotting analysis of synaptophysin expression in the adult + leptin group, adult + saline group, aged + leptin group and aged + saline group.

Article Snippet: Recombinant mouse leptin (Elabscience.

Techniques: Western Blot, Expressing, Saline

Journal: Degenerative Neurological and Neuromuscular Disease

Article Title: Neuroprotective Effects of Leptin on the APP/PS1 Alzheimer’s Disease Mouse Model: Role of Microglial and Neuroinflammation

doi: 10.2147/dnnd.s427781

Figure Lengend Snippet: Figure 3 Semi-quantitative analysis of synaptophysin expression in the adult + leptin group, adult + saline group, aged + leptin group and aged + saline group.

Article Snippet: Recombinant mouse leptin (Elabscience.

Techniques: Expressing, Saline

Journal: Degenerative Neurological and Neuromuscular Disease

Article Title: Neuroprotective Effects of Leptin on the APP/PS1 Alzheimer’s Disease Mouse Model: Role of Microglial and Neuroinflammation

doi: 10.2147/dnnd.s427781

Figure Lengend Snippet: Figure 4 Leptin increased microglial cell number in the hippocampus of adult mice, but not aged mice. (A–D) Representative images of Iba-1+ cells, in the hippocampus sections of the mice. (E–G) Quantitative analysis of Iba-1+ stained cells.

Article Snippet: Recombinant mouse leptin (Elabscience.

Techniques: Staining

Journal: Degenerative Neurological and Neuromuscular Disease

Article Title: Neuroprotective Effects of Leptin on the APP/PS1 Alzheimer’s Disease Mouse Model: Role of Microglial and Neuroinflammation

doi: 10.2147/dnnd.s427781

Figure Lengend Snippet: Figure 5 Influence of leptin on the levels of IL-1β (Left), IL-6 (Right) in the adult + leptin group, adult + saline group, aged + leptin group and aged + saline group.

Article Snippet: Recombinant mouse leptin (Elabscience.

Techniques: Saline

Journal: Degenerative Neurological and Neuromuscular Disease

Article Title: Neuroprotective Effects of Leptin on the APP/PS1 Alzheimer’s Disease Mouse Model: Role of Microglial and Neuroinflammation

doi: 10.2147/dnnd.s427781

Figure Lengend Snippet: Figure 6 Western blotting analysis of leptin receptor expression in the adult + leptin group, adult + saline group, aged + leptin group and aged + saline group.

Article Snippet: Recombinant mouse leptin (Elabscience.

Techniques: Western Blot, Expressing, Saline

Journal: Degenerative Neurological and Neuromuscular Disease

Article Title: Neuroprotective Effects of Leptin on the APP/PS1 Alzheimer’s Disease Mouse Model: Role of Microglial and Neuroinflammation

doi: 10.2147/dnnd.s427781

Figure Lengend Snippet: Figure 7 Semi-quantitative analysis of leptin receptor expression in the adult + leptin group, adult + saline group, aged + leptin group and aged + saline group.

Article Snippet: Recombinant mouse leptin (Elabscience.

Techniques: Expressing, Saline